Listeria monocytogenes phosphatidylinositol-specific phospholipase C: activation and allostery
摘要：
The animal and human pathogen Listeria monocytogenes secretes several virulence factors, including a phosphatidylinositol-specific phospholipase C (PI-PLC). Sufficient quantities of L. monocytogenes PI-PLC for biophysical studies were obtained by overexpression of the enzyme in Escherichia coli. The purified PI-PLC was examined in enzyme kinetics experiments using a new fluorogenic substrate, methyl-FLIP. Methyl-FLIP is a water-soluble monomeric substrate cleaved in a manner similar to the natural aggregate substrate, phosphatidylinositol (PI). Michaelis–Menten kinetics were observed with K
展开
关键词：
Enzyme kinetics Interfacial activation Phosphatidylinositol-specific phospholipase C (PI-PLC) Co-operativity
DOI：
10.1016/S0301-4622(02)00158-8
被引量：
年份：
2002
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
