The Tetrahymena ribozyme acts like an RNA restriction endonuclease
摘要：
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences.
展开
关键词：
Animals RNA, Catalytic RNA, Ribosomal Base Sequence Substrate Specificity Kinetics Mutation RNA Splicing Binding Sites Tetrahymena
DOI：
10.1038/324429a0
被引量：
年份：
1986
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
