Cloning and expression of mouse integrin beta p(beta 7): a functional role in Peyer's patch-specific lymphocyte homing

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作者：

Hu, M. C，Crowe, D. T，Weissman, I. L，B Holzmann

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摘要：

Lymphocytes express integrin receptors, termed lymphocyte Peyer's patch high endothelial venule (HEV) adhesion molecules (LPAMs), that mediate their organ-specific adhesion to specialized HEVs found in mucosal lymphoid organs (Peyer's patches). LPAM-1 consists of a murine integrin α4noncovalently associated with integrin βp. Here, we describe the cloning and expression of a mouse cDNA encoding βp, which is an 806-amino acid transmembrane glycoprotein. The genomic Southern blot analysis indicates that βpis the murine homologue of human β7. The function of α4β7as a Peyer's patch-specific adhesion molecule was tested directly by expression of the murine β7cDNA in an α4+β7-B-cell line or coexpression of the α4and β7cDNAs in an α4-β7-T-cell line. The transfected cells exhibited a new Peyer's patch-specific adhesive phenotype that could be specifically blocked by monoclonal antibodies against α4and β7. Moreover, an anti-β7monoclonal antibody specifically blocked binding of normal lymphocytes to Peyer's patch HEV but did not inhibit their binding to peripheral lymph node HEVs, indicating that β7is a unique component of the Peyer's patch-specific homing receptor.

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关键词：

Immunology Adhesion Molecule Cell Adhesion DNA Sequence High Endothelial Venule

DOI：

10.1073/pnas.89.17.8254

被引量：

498

年份：

1992