Synthesis of human fibroblast interferon by E. coli
摘要：
A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.
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关键词：
Fibroblasts Humans Escherichia coli Interferons DNA, Recombinant RNA, Messenger Cloning, Molecular Nucleic Acid Hybridization Protein Biosynthesis Transcription, Genetic
DOI：
10.1093/nar/8.18.4057
被引量：
年份：
1980
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