STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM IV. : Production and Purification of Type C Toxin for Conversion to Toxoid
摘要：
The medium found most suitable for production of toxin by C strains of Cl. botulinum was a solution of proteose peptone (Difco) 4%, with 2% of pancreatic casein digest (N-Z-Amine-type B, Sheffield Farms, Norwich, New York), 2% yeast extract (Baltimore Biological Laboratories) and 1% of glucose. Growth was for 5 days at 33°C. during which the initial pH of 7.6 had fallen to 5.5-6.0. The average ...
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关键词：
animal models antigens disease models formaldehyde human diseases immunity purification saturation strains temperature
DOI：
10.1128/JB.75.3.360-365.1958
被引量：
年份：
1958
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