Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1.
摘要：
Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A- population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.
展开
关键词：
Bone Marrow Humans Adipose Tissue Muscle, Smooth Fibroblasts Bone Marrow Cells Hematopoietic Stem Cells Cell Line Leukemia Immunoglobulin M
DOI：
10.1007/BF01714964
被引量：
年份：
1991
Semantic Scholar
(全网免费下载)
万方医学
Semantic Scholar
dx.doi.org
BioOne
查看更多
Europe PMC
NCBI
Oxford Univ Press
NCBI
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
ijaaonline.com
mysciencework.com
jimmunol.org
mendeley.com
scindeks.ceon.rs
mayoclinicproceedings.org
ajpendo.physiology.org
SAGE
haematologica.org
fertstert.org
jcb.rupress.org
cghjournal.org
rsif.royalsocietypublishing.org
bloodjournal.hematologylibrary.org
jacionline.org
pubfacts.com
bloodjournal.org
bloodjournal.org
(全网免费下载)
scienceopen.com
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
