Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA

来自 NCBI

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120

作者：

M Misrahi，M Atger，L D'Auriol，H Loosfelt，E Milgrom

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摘要：

A λgt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).

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关键词：

PR progesterone receptor progesterone receptor ER estrogen receptor estrogen receptor GR glucocorticoid receptor glucocorticoid receptor r rabbit rabbit h human human