Development of Versatile Metal-Organic Framework Functionalized Magnetic Graphene Core-Shell Biocomposite for Highly Specifically Recognizing Glycopeptides

来自 ACS

阅读量:

155

作者:

J WangJ LiY WangM GaoX ZhangP Yang

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摘要:

Protein N-glycosylation is a ubiquitous and important post-translational modification that has been involved in the development and progression of a series of human related diseases. Until recently, the highly selective capturing of glycopeptides from complex bio-sample is still significant and challenging work due to their changeable structures, ultra-low abundance and strong ion suppress effect. Here we firstly reported the preparation and characterization of a novel metal-organic frameworks (MOFs)-functionalized magnetic graphene hydrophilic porous biocomposite (MG@Zn-MOFs) for recognizing glycopeptides. Thanks to its strong magnetic responsiveness, large specific surface area, excellent biocompatibility and unique size-exclusion effect, the MG@Zn-MOFs showed outstanding sensitivity and selectivity and good recyclability in glycopeptides analysis. More excitingly, in practical application, 517 N-glycopeptides within 151 unique glycoproteins were clearly identified from the human serum (1μL) treated with the MG@Zn-MOFs, which is the best result among published reports so far. All the results demonstrate the promising of the biocomposite for the commercialized usage in the enrichment of glycopeptides in complex sample through the convenient and efficient process. Furthermore, it is anticipated that our strategy may offer promising guidance to develop new bio-MOFs functionalized biocomposite for glycoproteomics applications.

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DOI:

10.1021/acsami.6b08218

被引量:

4

年份:

2016

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