Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo
摘要：
The Syrinx 2A phage and π AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified λ gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.
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DOI：
10.1073/pnas.84.13.4379
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年份：
1987
Semantic Scholar
万方医学
掌桥科研
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