摘要：

A capillary electrophoresis method for chiral separation of citalopram was established using sulfated-β-cyclodextrin as the chiral additive.Several factors on the chiral separation,including the composition,concentration and pH of buffer and the type and concentration of chiral selectors have been studied.The optimized condition was obtained at 0.04%(w/w)sulfated-β-cyclodextrin in 20 mmol/L citric acid.Samples were injected into capillary at a migration stress of 0.5psi and injection time of 5seconds.Separation voltage was+20kV and detection wavelength was 205 nm.The results showed that the detection limits of both enantiomers were 90μg/L.The relative standard deviations of migration time and peak area were less than 3.0%.Calibration curve was linear over the range of 0.05-100mg/L,and the correlation coefficient was larger than 0.998.This method was successfully used to determine Escitalopram medicine precisely,with average recoveries between 103% and 108%.

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