Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria
摘要：
Microdomains of high intracellular calcium ion concentration, [Ca$^{2+}$]$_i$, have been hypothesized to occur in living cells exposed to stimuli that generate inositol 1,4,5-trisphosphate (IP$_3$). Mitochondrially targeted recombinant aequorin was used to show that IP$_3$-induced Ca$^{2+}$ mobilization from intracellular stores caused increases of mitochondrial Ca$^{2+}$ concentration, [Ca$^{2+}$]$_m$, the speed and amplitude of which are not accounted for by the relatively small increases in mean [Ca$^{2+}$]$_i$. A similar response was obtained by the addition of IP$_3$ to permeabilized cells but not by perfusion of cells with Ca$^{2+}$ at concentrations similar to those measured in intact cells. It is concluded that in vivo, domains of high [Ca$^{2+}$]$_i$ are transiently generated close to IP$_3$-gated channels and sensed by nearby mitochondria; this may provide an efficient mechanism for optimizing mitochondrial activity upon cell stimulation.
展开
关键词：
Humans Mitochondria Hela Cells Inositol 1,4,5-Trisphosphate Calcium Histamine Recombinant Fusion Proteins Aequorin Cell Membrane Permeability Osmolar Concentration
DOI：
10.1126/science.8235595
被引量：
年份：
1993
Taylor & Francis
dx.doi.org
NCBI
Oxford Univ Press
JSTOR
查看更多
Europe PMC
NCBI
NRC Research Press
AACR
JBC
dialnet.unirioja.es
adsabs.harvard.edu
ResearchGate
ProQuest
EureKaMag.com
(全网免费下载)
EBSCO
EBSCO
mysciencework.com
onAcademic
mendeley.com
diabetes.diabetesjournals.org
ajpcell.physiology.org
joe.endocrinology-journals.org
ganino.com
(全网免费下载)
sciencemag.org
(全网免费下载)
jgp.rupress.org
highbeam.com
jvi.asm.org
jp.physoc.org
molbiolcell.org
circ.ahajournals.org
rstb.royalsocietypublishing.org
jneurosci.org
scienceopen.com
emboj.embopress.org
elifesciences.org
deepdyve.com
bioscirep.org
jcs.biologists.org
jpet.aspetjournals.org
www.socolar.com
go.galegroup.com
genesdev.cshlp.org
sciencemag.org
biochemsoctrans.org
fasebj.org
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
