Phytohaemagglutinin activation of T cells through the sheep red blood cell receptor

来自 EBSCO

阅读量：

作者：

O\"Flynn, Kieran， Krensky, Alan M.， Beverley, Peter C. L.， Burakoff, Steven J.， Linch, David C.

展开

摘要：

Expression of receptors for sheep red blood cells and the ability to proliferate in response to phytohaemagglutinin (PHA) are the traditional properties of human T cells 1,2 , but the function of the sheep red cell receptor (the T11 antigen) is controversial 3,4 and the mechanism of PHA-induced mitogenesis unclear. Mitogenesis involves a complex series of cell-mediated and factor-dependent interactions, but a rise in intracellular free calcium concentration, [Ca 2+ ] i , seems to be an important primary event in T-cell activation 5–7 . We have now investigated the effects of three monoclonal antibodies, previously shown to inhibit mitogen-induced proliferation 3,8,9 , on T-cell [Ca 2+ ] i . We find that anti-LFA-2 and OKT11, which react with the sheep red cell receptor 8,10 , have no effect on [Ca 2+ ] i , nor do they inhibit the rise in [Ca 2+ ] i induced by concanavalin A (Con A) or the mitogenic anti-T3 monoclonal antibody UCHT1 (ref. 11). They do, however, block PHA-induced Ca 2+ mobilization. Anti-LFA-1, which reacts with the lymphocyte function-associated antigen 8,12 , has no effect on intracellular Ca 2+ . These studies suggest that the sheep red blood cell receptor is an activation pathway for T cells and that the effects of PHA are mediated through this pathway.

展开

DOI：

10.1038/313686a0

被引量：

427

年份：

1985