摘要：

A single exposure to raw kidney beans (RKB) results in vesiculation, shortening, and then regrowth of microvilli in the rat small intestine. This study investigated changes that occur in the structure of microvilli 2-10 hours after RKB exposure.Circumferences of microvilli from absorptive cells obtained sequentially after challenge with RKB or chow were assigned to one of three groups: small, intermediate, or large. The distribution and concentration of actin in intact mucosae or isolated epithelial sheets were determined by confocal laser scanning microscopy, immunocytochemistry, and immunoblot analysis with specific probes.Six hours after exposure to RKB, most microvilli were large, abnormal in shape, and contained significantly more actin filaments than large microvilli from control rats. In addition, the fluorescence intensity of F-actin increased within injured microvilli without changes in the total intracellular actin concentration. By 8-10 hours after challenge with RKB, some microvilli remained larger than those of control rats but had resumed their normal shape and contained fewer actin filaments than at 6 hours.Exposure of the rat small intestine to RKB results in enlargement of absorptive cell microvilli and reorganization of membrane and core actin filaments without changes in intracellular actin concentration. Enlarged microvilli are rapidly repaired.

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