Nanostructures for enzyme stabilization
摘要:
Recent breakthroughs in nanotechnology have made various nanostructured materials more affordable for a broader range of applications. Although we are still at the beginning of exploring the use of these materials for biocatalysis, various nanostructures have been examined as hosts for enzyme immobilization via approaches including enzyme adsorption, covalent attachment, enzyme encapsulation, and sophisticated combinations of methods. This review discusses the stabilization mechanisms behind these diverse approaches; such as confinement, pore size and volume, charge interaction, hydrophobic interaction, and multipoint attachment. In particular, we will review recently reported approaches to improve the enzyme stability in various nanostructures such as nanoparticles, nanofibers, mesoporous materials, and single enzyme nanoparticles (SENs). In the form of SENs, each enzyme molecule is surrounded with a nanometer scale network, resulting in stabilization of enzyme activity without any serious limitation for the substrate transfer from solution to the active site. SENs can be further immobilized into mesoporous silica with a large surface area, providing a hierarchical approach for stable, immobilized enzyme systems for various applications, such as bioconversion, bioremediation, and biosensors.
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关键词:
Practical/ biotechnology catalysis enzymes nanoparticles nanotechnology/ nanostructured materials enzyme stabilization nanotechnology biocatalysis enzyme immobilization enzyme adsorption covalent attachment enzyme encapsulation charge interaction hydrophobic interaction multipoint attachment pore size nanofibers mesoporous materials single enzyme nanoparticles enzyme molecule mesoporous silica immobilized enzyme systems bioconversion bioremediation biosensors/ E3644T Nanotechnology industry E3628 Biotechnology industry E1710 Engineering materials
DOI:
10.1016/j.ces.2005.05.067
被引量:
年份:
2006
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