Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
申请(专利)号：
06/697808
申请日期：
Feb 4, 1985
公开/公告号：
US4769061
公开/公告日期：
Sep 6, 1988
申请(专利权)人：
Calgene Inc. (Davis, CA)
被引量：
摘要：
Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3- phosphoshikimate synthase (EC: 2.5.1.19) (ES-3-P synthase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme. The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and given A.T.C.C. Accession No. 39256.
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