Identification and characterization of a nuclear pore complex protein
摘要：
We describe studies using a monoclonal antibody that recognizes a 62 kd protein (p62) of rat liver nuclei. This protein remains associated with the nuclear pore complex-lamina fraction resulting from treatment of nuclei with DNAase, RNAase, and nonionic detergent. Immunofluorescence revealed a strikingly punctate pattern of nuclear rim staining. By immunoferritin microscopy, p62 was specifically localized to the pore complex. Thus, pore complexes can be resolved by fluorescence light microscopy. Pulse chase analysis of labeled tissue culture cells showed that p62 is synthesized as a soluble cytoplasmic precursor of 61 kd, which is incorporated into the nuclear fraction with an unusually long t 1 2 of about 6 hr. Incorporation is followed by modification that may involve addition of N-acetylglucosamine residues.
展开
关键词：
Humans Hematopoietic Stem Cells Breast Neoplasms Biological Markers Antigens, CD34 Antibodies, Monoclonal Reproducibility of Results Predictive Value of Tests Immunomagnetic Separation Adult
DOI：
10.1016/0092-8674(86)90784-1
被引量：
年份：
1986
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
