摘要：

Summary This study assayed glucose utilization via the hexose monophosphate (HMP) shunt and Embden-Meyerhof pathways in lymphocytes from patients with diabetes mellitus (DM) and chronic lymphocytic leukaemia (CLL) employing the quantitative evolution of 14CO2 and production of radioactive glycolytic intermediates from [1-14C]glucose as respective experimental endpoints. In addition, DNA synthesis, as reflected by [14C]thymidine incorporation by the same cells in tissue culture, was measured. Most diabetic lymphocytes displayed depressed absolute and proportional glucose metabolism through the direct oxidative pathway and also demonstrated decreased incorporation of radioactive thymidine, particularly when the lymphocytes were stimulated by phytohaemagglutinin (PHA). The lymphocytes in chronic lymphocytic leukaemia showed the same abnormalities. Moreover, in all lymphocytes assayed, a correlative, semiquantitative relationship was present betw

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