Structure and processing of yeast precursor tRNAs containing intervening sequences
摘要：
We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.
展开
关键词：
Saccharomyces cerevisiae Tyrosine Tyrosine-tRNA Ligase RNA Ligase (ATP Anticodon Base Sequence Nucleic Acid Conformation Nucleic Acid Precursors RNA, Transfer
DOI：
10.1038/274438a0
被引量：
年份：
1978
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
