A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
摘要：
Extracts of Hemophilus influenzae strain Rd contain an endonuclease activity which produces a rapid decrease in the specific viscosity of a variety of foreign native DNA's; the specific viscosity of H. influenzae DNA is not altered under the same conditions. This "restriction" endonuclease activity has been purified approximately 200-fold. The purified enzyme contains no detectable exo- or endonucleolytic activity against H. influenzae DNA. However, with native phage T7 DNA as substrate, it produces about 40 double-strand 5′-phosphoryl, 3′-hydroxyl cleavages. The limit product has an average length of about 1000 nucleotide pairs and contains no single-strand breaks. The enzyme is inactive on denatured DNA and it requires no special co-factors other than magnesium ions.
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关键词：
chromosome aberrations congenital abnormalities Down's syndrome genetic disorders human diseases mental retardation sex chromosomes
DOI：
10.1016/0022-2836(70)90149-X
被引量：
年份：
1970
Taylor & Francis
Elsevier
万方医学
Semantic Scholar
dx.doi.org
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