Evidence for the stochastic integration of gene trap vectors into the mouse germline.
摘要：
A large scale insertional experiment was performed in stem (ES) cells by introducing two types of gene trap vectors into the genome. These cell lines carrying mutations were introduced into the germline. In order to assess the feasibility of a large scale cloning of the targeted genes from these lines, we have isolated and characterized 55 trapped exons from the corresponding ES cells. Analysis of the data has revealed that vectors containing or lacking an internal entry site (IRES) can integrate into the ES cell genome stochastically. The targeted genes comprise 30% known genes, 20% expressed sequence tags (ESTs) and 50% novel or unknown genes. The known genes belong to several major classes and represent complete or partial knockouts. Using currently available methods or modifications of them, it should be feasible to do a large scale cloning of trapped genes from the ES cell lines.
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关键词：
Animals Cattle Humans Mice Ribosomes Cell Line Enzymes Proteins Recombinant Proteins Cloning, Molecular
DOI：
10.1093/nar/25.8.1531
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