The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies

来自 EBSCO

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189

作者：

D.，H.，Lunt，D.-X.，Zhang，J.，M.，Szymura，O.，M.

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摘要：

Insect mitochondrial cytochrome oxidase I (COI) genes are used as a model to examine the within-gene heterogeneity of evolutionary rate and Its implications for evolutionary analyses. The complete sequence (1537 bp) of the meadow grasshopper (Chorthippus parallelus) COI gene has been determined, and compared with eight other Insect COI genes at both the DNA and amino acid sequence levels. This reveals that different regions evolve at different rates, and the patterns of sequence variability seems associated with functional constraints on the protein. The COOH-terminal was found to be significantly more variable than Internal loops (I), external loops (E), transmembrane helices (M) or the NH2 terminal. The central region of COI (M5-M8) has lower levels of sequence variability, which Is related to several Important functional domains In this region. Highly conserved primers which amplify regions of different variabilities have been designed to cover the entire insect COI gene. These prim

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关键词：

insect Chorthlppus parallelus cytochrome oxidase I mitochondrial DNA conserved PCR primers genetic marker

DOI：

10.1111/j.1365-2583.1996.tb00049.x

被引量：

1102

年份：

1996