Synthesis of enantiopure fluorohydrins using alcohol dehydrogenases at high substrate concentrations.
摘要：
The use of purified and overexpressed alcohol dehydrogenases to synthesize enantiopure fluorinated alcohols is shown. When the bioreductions were performed with ADH-A from Rhodococcus ruber overexpressed in E. coli, no external cofactor was necessary to obtain the enantiopure (R)-derivatives. Employing Lactobacillus brevis ADH, it was possible to achieve the synthesis of enantiopure (S)-fluorohydrins at a 0.5 M substrate concentration. Furthermore, due to the activated character of these substrates, a huge excess of the hydrogen donor was not necessary.
展开
关键词：
alcohols (benzene compounds reduction, hydrogenation biochemical syntheses, microbiological syntheses diastereoselective syntheses, enantioselective syntheses (incl. cis/trans‐isomerism
DOI：
10.1021/jo400962c
被引量：
年份：
2013
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
