摘要：

SUMMARYElevation of intracellular cAMP levels has been shown previously to inhibit cytokine secretion by various cell types in vitro. Since salmeterol is a β2-agonist which activates adenylate cyclase, its ability to inhibit cytokine production was evaluated. Though salmeterol, and the related drug albuterol, did not inhibit IL-1β production in vitro, both drugs did inhibit tumour necrosis factor-alpha (TNF-α) secretion by lipopolysaccharide (LPS)-activated THP-1 cells with similar IC50S of approximately 0·1μm. This inhibition was effectively reversed by the β2-antagonist oxprenolol, indicating that the inhibition was mediated through the β2-adrenergic receptor. A strikingly different reactivity profile was seen with T cells. Salmeterol was able to inhibit the activation of both mouse and human T cells, as measured by proliferation and IL-2 secretion in response to anti-CD3 antibody, whereas albuterol was c

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