摘要：

Drug sensitivity assays were performed using a variation of a colori metrie [3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)1 assay on V79, CHO-AuxBl, CH"C5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with data assimi lated simultaneously by clonogenic assay and by dye exclusion assay. Good correlation was observed using the CHO-AuxBl cell line and the pleiotropic drug-resistant mutant CH"C5, with similar degrees of relative resistance observed with both the M l'I and clonogenic assays. Good correlation was observed between the clonogenic and M l'I assays for I-h drug exposures, although the MIT assay was more sensitive to vinblastine. In general, the clonogenic assay was more sensitive when continuous drug exposures were utilized, although this was primarily related to the increased drug exposure time. While the use of the MIT

展开