Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts

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摘要：

Expressed sequence tag (EST) libraries for , the causative agent of green muscardine disease, were developed from the broad -range pathogen sf. anisopliae and the specific grasshopper pathogen, sf. acridum. Approximately 1,700 5' end sequences from each subspecies were generated from cDNA libraries representing grown under conditions that maximize of cuticle-degrading enzymes. Both subspecies had ESTs for virtually all pathogenicity-related genes cloned to date from , but many novel genes encoding potential virulence factors were also tagged. Enzymes with potential targets in the insect included , , phospholipases, , , and enzymes producing toxic . A diverse array of composed 36 % of all sf. anisopliae ESTs. Eighty percent of the ESTs that could be clustered into functional groups had significant matches (E<10(-5)) in other ascomycete . These included genes reported to have specific roles in pathogens with plant or vertebrate . Many of the remaining ESTs had their best BLAST match among animal, plant and bacterial sequences. These include genes with plant and microbial counterparts that produce potent antimicrobials. The abundance of transcripts discovered for different functional groups varied between the two subspecies of in a manner consistent with ecological adaptations of the two pathogens. By hastening gene discovery this project has enhanced of improved mycoinsecticides. In addition, the ESTs represent a significant contribution to the extensive database of sequences from that are saprophytes or plant and vertebrate pathogens. Comparative analyses of these sequences is providing important information about the biology and evolutionary history of this clade.

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DNA biology computing image processing molecular biophysics pipeline processing DNA microarray image processing modular structure six-stage pipeline Bioinformatics DNA