Commitment of B Lymphocytes to a Plasma Cell Fate Is Associated with Blimp-1 Expression In Vivo

来自掌桥科研

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106

作者：

C Angelin-Duclos，G Cattoretti，KI Lin，K Calame

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摘要：

B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor that is sufficient to trigger terminal differ,entiation in the B cell lymphoma BCL-1.In this study,we have determined the expression pattern of Blimp-1 in vivo in either a T-independent or T-dependent response in plasma cells that have undergone isotype switching and those resulting from secondary immunization.Blimp-1 is also present in long-lived plasma cells residing in the bone marrow.However,Blimp-1 was not detected in memory B cells.This expression pattern provides further evidence of a critical role for Blimp-1 in plasma cell development,supporting earlier studies in cultured lines.Significantly,Blimp-1 was also found in a fraction (4-15%) of germinal centre B cells in murne spleen and human tonsils.Blimp-1 expression in the germinal center is associated with an interesting subset of cells with a phenotype intermediate between germinal center B cells and plasma cells.In the mouse,Blimp-1~+ germinal center B cells peak at day 12 postimmunization and sisappear soon thereafter.They are not apoptotic,some are proliferating,they express germinal center markers peanut agglutinin or CD10 but not Bcl-6,and most express CD138 (syndecan-1),IRF4,and cytoplasmic Ig,Together,these data support a model in which B cell fate decisions occur within the germinal center and Blimp-1 expression is critical for commitment to a plasma cell,rather than a memory cell,fate.

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关键词：

Animals Mice, Inbred C57BL Humans Mice Germinal Center Bone Marrow Cells Phenylacetates Transcription Factors Repressor Proteins Ficoll