The role of DNA topoisomerases in recombination and genome stability: A double-edged sword?

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Although androgens are believed to influence the distribution of human adipose tissue and have been detected in human fat, receptors for these sex hormones have yet to be identified. These studies demonstrate that a high-affinity, limited-capacity binding component for the synthetic androgen methyltrienolone (R1881) exists in ammonium sulfate precipitates of human adipose tissue cytosols. The equilibrium dissociation constant (K(d) = 0.1 to 0.4 nmol/L, n = 6) and the number of binding sites (2 to 26 fmol/mg protein, n = 22) are consistent with those reported for androgen receptors in rat prostate, human prostatic carcinoma, MCF-7 cells, and baboon myocardium. The relative steroid-binding specificities of the human adipose tissue androphile (R1881 approximately 5-alpha-dihydrotestosterone > testosterone > estradiol approximately progesterone >> dexamethasone) are similar, but not identical, to those reported for androgen receptors in rat prostate (R1881 > 5-alpha-dihydrotestosterone approximately > testosterone > estradiol > progesterone >> cortisol) and baboon myocardium (R1881 > 5-alpha-dihydrotestosterone > testosterone > progesterone > estradiol >> cortisol). The function of the androgen-binding component in human adipose tissue is not known.

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Saccharomyces cerevisiae Escherichia coli DNA Topoisomerases, Type I DNA Topoisomerases, Type II Sequence Homology, Nucleic Acid DNA Replication Recombination, Genetic Amino Acid Sequence Molecular Sequence Data Genes