EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway

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作者：

B Neumann，S Coakley，R Giordano-Santini，C Linton，ES Lee，A Nakagawa，D Xue，MA Hilliard

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摘要：

Functional after nervous system injury requires transected to reconnect with their original target tissue. Axonal fusion, a spontaneous regenerative mechanism identified in several species, provides an efficient means of achieving target reconnection as a regrowing is able to contact and fuse with its own separated fragment, thereby re-establishing the original axonal tract. Here we report a molecular characterization of this process in , revealing dynamic changes in the subcellular of the EFF-1 fusogen after axotomy, and establishing (PS) and the PS receptor (-1) as critical components for axonal fusion. -1 functions -autonomously in the regrowing neuron and, instead of acting in its canonical pathway, acts in a parallel phagocytic pathway that includes the -52, as well as CED-7, -5 and CED-6 (refs 9, 10, 11, 12). We show that -52 binds to PS exposed on the injured , and can restore fusion several hours after injury. We propose that PS functions as a 'save-me' signal for the distal fragment, allowing conserved molecules to function in re-establishing axonal integrity during of the nervous system.

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关键词：

Regeneration and repair in the nervous system Apoptosis

DOI：

10.1038/nature14102

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年份：

2015