Effects of bone morphogenetic protein‐7 (BMP‐7) on primordial follicular growth in the mouse ovary

来自 EBSCO

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作者：

Woo‐Sik Lee，Se‐Jin Yoon，Tae‐Ki Yoon，Kwang‐Yul Cha，Sook‐Hwan Lee，S Shimasaki，S Lee，Kyung‐Ah Lee

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摘要：

Previously, bone morphogenetic protein-7 (BMP-7) was suggested as a factor that may act to facilitate the transition of follicles from primordial stage to the pool of developed primary, preantral, and antral follicles (Lee et al. 2001: Biol Reprod 65:994–999.). Thus, aim of the present study was to evaluate effect(s) of BMP-7 on the primordial-primary follicle transition. Neonatal mouse ovaries were cultured in the presence or absence of 100 mIU/ml FSH with various doses of BMP-7 (0, 10, and 100 ng/ml). After 4-day culture period, number of follicles was counted and the expression of transcripts for FSH receptor (FSHR), kit ligand (KL), and c-kit was measured by RT-PCR. BMP-7 alone at 100 ng/ml concentration stimulated follicle development with concurrent increase of mRNA for FSHR. BMP-7 alone down-regulated KL expression however, the ratio between KL1 and KL2 was increased. There was no change in the c-kit mRNA expression. Results of the present study suggest that the BMP-7 is one of the factors involved in primordial-primary follicle transition in the mouse ovary and it may play a role in expression of FSHR for further follicular development. Mol. Reprod. Dev. 69: 159–163, 2004. 2004 Wiley-Liss, Inc.

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关键词：

mouse BMP‐7 primordial‐primary follicle transition c‐kit kit ligand

DOI：

10.1002/mrd.20163

被引量：

210

年份：

2004