Human β-Defensin-1 Is a Salt-Sensitive Antibiotic in Lung That Is Inactivated in Cystic Fibrosi
摘要：
A human bronchial xenograft model was used to characterize the molecular basis for the previously described defect in bacterial killing that is present in the cystic fibrosis (CF) lung. Airway surface fluid from CF grafts contained abnormally high NaCl and failed to kill bacteria, defects that were corrected with adenoviral vectors. A full-length clone for the only known human β-defensin (i.e., hBD-1) was isolated. This gene is expressed throughout the respiratory epithelia of non-CF and CF lungs, and its protein product shows salt-dependent antimicrobial activity to P. aeruginosa. Antisense oligonucleotides to hBD-1 ablated the antimicrobial activity in airway surface fluid from non-CF grafts. These data suggest that hBD-1 plays an important role in innate immunity that is compromised in CF by its salt-dependent inactivation.
展开
关键词：
Humans Cystic Fibrosis beta-Defensins Antisense Elements (Genetics Oligonucleotide Probes Blood Proteins Anti-Bacterial Agents Base Sequence Amino Acid Sequence Molecular Sequence Data
DOI：
10.1016/S0092-8674(00)81895-4
被引量：
年份：
1997
Elsevier
Elsevier
(全网免费下载)
BMJ
万方医学
Semantic Scholar
(全网免费下载)
查看更多
dx.doi.org
Europe PMC
NCBI
Cell Press
Cell Press
(全网免费下载)
The Lancet
ResearchGate
EBSCO
EBSCO
iai.asm.org
science-open.com
columbia.edu
(全网免费下载)
core.ac.uk
(全网免费下载)
deepdyve.com
columbia.edu
(全网免费下载)
jacionline.org
perspectivesinmedicine.cshlp.org
infona.pl
ajpcell.physiology.org
ajog.org
err.ersjournals.com
mysciencework.com
scindeks.ceon.rs
scienceopen.com
plantphysiol.org
Elsevier
(全网免费下载)
Semantic Scholar
(全网免费下载)
Cell Press
(全网免费下载)
columbia.edu
(全网免费下载)
columbia.edu
(全网免费下载)
core.ac.uk
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
