Oin methanol for 30 minutes to abolish the endogenous peroxidase activity. Sections were further blocked with 10 mL/L normal goat serum for 0.5 hour, followed by incubation with polyclonal rabbit anti-APMCF1 serum (1:100) at 4 overnight. The sections were
摘要：
AIM: APMCF1 is a novel human gene whose transcripts are up-regulated in apoptotic MCF-7 cells. In order to learn more about this gene's function in other tumors, we cloned its full length cDNA and prepared its polyclonal antibody to investigate its expression in colon cancers with immunohistochemistry.
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