Analysis of radioligand binding experiments. A collection of computer programs for the IBM PC

阅读量:

107

作者:

GA Mcpherson

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摘要:

It is now well accepted that graphic methods are a poor choice of analytical technique for analyzing radioligand binding data given the complexity of some radioligand-binding site interactions; this is true not only for studies performed at equilibrium but also for those examining kinetic properties of the radioligand. As a consequence the computer plays an important role in all forms of radioligand binding experiments because it reduces the labor-intensive calculations associated with such experiments and it allows the use of more appropriate weighted nonlinear curve-fitting techniques that more accurately describe the radioligand-binding site interaction. This paper describes a collection of four programs ( klnetic, ebda, ligad, and lowry) that can be used to analyze most forms of radioligand binding experiments. The programs have been adapted for use on an IBM PC, which is a popular, inexpensive microcomputer and which is well suited to the analysis of radioligand binding data.

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DOI:

10.1016/0160-5402(85)90034-8

被引量:

2474

年份:

1985

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1995
被引量:207

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