The intervening sequence RNA of Tetrahymena is an enzyme
摘要：
A shortened form of the self-splicing ribosomal RNA (rRNA) intervening sequence of Tetrahymena thermophila acts as an enzyme in vitro. The enzyme catalyzes the cleavage and rejoining of oligonucleotide substrates in a sequence-dependent manner with Km = 42 microM and kcat = 2 min-1. The reaction mechanism resembles that of rRNA precursor self-splicing. With pentacytidylic acid as the substrate, successive cleavage and rejoining reactions lead to the synthesis of polycytidylic acid. Thus, the RNA molecule can act as an RNA polymerase, differing from the protein enzyme in that it uses an internal rather than an external template. At pH 9, the same RNA enzyme has activity as a sequence-specific ribonuclease.
展开
DOI：
10.1126/science.3941911
被引量：
年份：
1986
万方医学
Semantic Scholar
dx.doi.org
NCBI
JSTOR
查看更多
NCBI
Europe PMC
Oxford Univ Press
adsabs.harvard.edu
ResearchGate
ProQuest
EBSCO
www.socolar.com
rnajournal.cshlp.org
mendeley.com
uregina.ca
highbeam.com
mysciencework.com
physiolgenomics.physiology.org
clinsci.org
onAcademic
sciencemag.org
rspb.royalsocietypublishing.org
aac.asm.org
molpharm.aspetjournals.org
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
