Abundance and DNA sequence of two-base repeat regions in tropical tree genomes.

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摘要：

Tandem DNA repeats of two-base pairs are potentially important tools for population genetic studies because of their abundance and length variation. As part of our research into the ecology of tropical forest , we began a study of repeat regions in several genera of tropical trees. Genomic libraries in were screened with the oligonucleotide probes poly(GT) and poly(AG). Both types of repeat regions were abundant in the genomes of all six plant species examined. Using the size of inserts in the phage libraries and number of phage screened, we estimated that there were 5 x 10(3) to 3 x 10(5) poly(AC) sites per genome, with slightly more AG than AC sites. When libraries were made from smaller fragments of genomic DNA, abundance estimates were higher, suggesting that two-base repeat sites were clustered in the genome. Poly(AC) sites were 16-22 bp in length, and four of the five sequenced were adjacent to either poly(AG)or poly(AT) sites. Other repeat region s appeared in DNA flanking the AC sites. This further demonstrated that two-base repeats and other repetitive DNA were clustered in the genome. Two-base repeats are abundant in plant genomes and could provide a large number of polymorphic markers for studies of plant population genetics.

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关键词：

population genetics, virola sebifera, Poulsenia armata, trophis racemosa, Moraceae, repetitive sequences, tandem dna repeats, piper reticulatum, genome, nucleotide sequences, malmea, Virola, Annonaceae, Piper, genetic markers

DOI：

10.1139/g91-011

被引量：

787

年份：

1991