Mutant loxP vectors for selectable marker recycle and conditional knock-outs
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42
摘要：
BackgroundGene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used as a model for gene knock-outs due to its high targeted integration activity. Disruption of multiple genes and complementation of the phenotypes is, however, restricted by the number of available selectable marker genes. It is therefore highly desirable to recycle the selectable markers using a site-specific recombination system like Cre/loxP.
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DOI：
10.1186/1472-6750-1-7
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年份：
2001
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Springer
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Semantic Scholar
(全网免费下载)
BioMed Central
(全网免费下载)
NCBI
(全网免费下载)
Pubmed Central
(全网免费下载)
Europe PMC
(全网免费下载)
BioMed Central
(全网免费下载)
BioMed Central
(全网免费下载)
EureKaMag.com
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biomedsearch.com
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core.ac.uk
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来源期刊
引用走势
2014
被引量：42
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