AID Enzyme-Induced Hypermutation in an Actively Transcribed Gene in Fibroblasts
摘要：
Activation-induced cytidine deaminase (AID), a putative RNA-editing enzyme, is indispensable for somatic hypermutation (SHM), class switch recombination, and gene conversion of immunoglobulin genes, which indicates a common molecular mechanism for these phenomena. Here we show that ectopic expression of AID alone can induce hypermutation in an artificial GFP substrate in NIH 3T3 murine fibroblast cells. The frequency of mutations was closely correlated with the level of transcription of the target gene, and the distribution of mutations in NIH 3T3 cells was similar to those of SHM in B lymphocytes. These results indicate that AID is sufficient for the generation of SHM in an actively transcribed gene in fibroblasts, as well as B cells, and that any of the required cofactors must be present in these fibroblasts.
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关键词：
B-Lymphocytes 3T3 Cells Animals Mice Cytidine Deaminase Luminescent Proteins Green Fluorescent Proteins Recombinant Proteins DNA Transfection
DOI：
10.1126/science.1071556
被引量：
年份：
2002
Wiley
万方医学
dx.doi.org
Oxford Univ Press
JSTOR
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