Solenoidal model for superstructure in chromatin
摘要：
Chromatin prepared by brief digestion of nuclei with micrococcal nuclease, and extracted in 0.2 mM EDTA, appears in the electron microscope as filaments of about 100 angstrom diameter which coil loosely. In 0.2 mM Mg++these ``nucleofilaments'' condense into a supercoil or solenoidal structure of pitch about 110 angstrom corresponding to the diameter of a nucleofilament. It is proposed that the x-ray reflections at orders of 110 angstrom observed in chromatin originate in the spacing between turns of the solenoid rather than that between nucleosomes along the nucleofilament. The solenoidal structure appears to need histone H1 for its stabilization. Under certain conditions, isolated nucleosomes can also aggregate into a similar structure. The solenoidal structure can be correlated with the ``thread'' of diameter about 300 angstrom observed by other workers in nuclei.
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DOI：
10.1073/pnas.73.6.1897
被引量：
年份：
1976
Taylor & Francis
掌桥科研
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