Substrate phage: Selection of protease substrates by monovalent phage display.

来自 EBSCO

阅读量:

28

作者:

MatthewsDavidJ.WellsJamesA.

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摘要:

Describes a method for identifying good protease substrates among millions of possible sequences. Construction of a library of fusion proteins; Display of each fusion protein as a single copy; How phages were bound to an affinity support and then treated with the protease of interest; How the phages with good protease substrates were identified; Example; Other possible applications of technique; Details.

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被引量:

298

年份:

1993

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来源期刊

引用走势

2011
被引量:25

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