Substrate phage: Selection of protease substrates by monovalent phage display.
摘要:
Describes a method for identifying good protease substrates among millions of possible sequences. Construction of a library of fusion proteins; Display of each fusion protein as a single copy; How phages were bound to an affinity support and then treated with the protease of interest; How the phages with good protease substrates were identified; Example; Other possible applications of technique; Details.
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年份:
1993
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2011
被引量:25
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