Modulation of GABAergic transmission by activity via postsynaptic Ca2+-dependent regulation of KCC2 function.
摘要：
Activity-induced modification of GABAergic transmission contributes to the plasticity of neural circuits. In the present work we found that prolonged postsynaptic spiking of hippocampal neurons led to a shift in the reversal potential of GABA-induced Cl − currents (E Cl) toward positive levels in a duration- and frequency-dependent manner. This effect was abolished by blocking cytosolic Ca 2+ elevation and mimicked by releasing Ca 2+ from internal stores. Activity- and Ca 2+-induced E Cl shifts were larger in mature neurons, which express the K-Cl cotransporter KCC2 at high levels, and inhibition of KCC2 occluded the shifts. Overexpression of KCC2 in young cultured neurons, which express lower levels of KCC2 and have a more positive E Cl, resulted in hyperpolarized E Cl similar to that of mature cells. Importantly, these young KCC2-expressing neurons became responsive to neuronal spiking and Ca 2+ elevation by showing positive E Cl shifts. Thus, repetitive postsynaptic spiking reduces the inhibitory action of GABA through a Ca 2+-dependent downregulation of KCC2 function.
展开
DOI：
10.1016/j.neuron.2005.10.025
被引量：
年份：
2005
Elsevier
(全网免费下载)
Elsevier
国家科技图书文献中心
(权威机构)
Semantic Scholar
(全网免费下载)
掌桥科研
查看更多
万方医学
dx.doi.org
Ingenta
Cell Press
(全网免费下载)
Europe PMC
Oxford Univ Press
NCBI
Cell Press
ProQuest
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
neuro.bcm.edu
(全网免费下载)
www.socolar.com
mysciencework.com
mendeley.com
core.ac.uk
(全网免费下载)
jn.physiology.org
pubfacts.com
Elsevier
(全网免费下载)
Semantic Scholar
(全网免费下载)
Cell Press
(全网免费下载)
ResearchGate
(全网免费下载)
core.ac.uk
(全网免费下载)
neuro.bcm.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
