摘要：

ml of epoxide hydrolase. For anthracene, reactions were terminated by addition of 1 ml of acetone, and metabolites were extracted with 2 ml of ethyl acetate. The organic layer wasremoved, dried over sodium sulfate, and concentrated in a stream of dry nitrogen. The residue was dissolved in 100 pl of methanol for subsequent analysis by HPLC. A 25-pl aliquot of each solution was injected onto a DuPont Golden series Zorbax ODS column (0.62 X 10 cm). The column was eluted with a linear gradient (3.3% per min) from 50 to 100% methanol in water at a flow rate of 1.6 ml/min. For naphthalene the incubation medium was saturated with sodium chloride and metabo- lites were extracted with 1ml of ethyl acetate/acetone (2:l). Due to volatility of naphthalene, this solution was not concentrated, and 30 plof each extract was injected directly onto a DuPont Zorbax ODS column (0.94 X 25 cm) eluted with a linear gradient (6% per min) of 40-100% methanol in water at a flow rate of2.8 ml/min. For both hydrocarbons, fractions were collected every 0.3 min and analyzed for radioactivity in Hydrofluor scintillation mixture.

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