A Fourier-transform infrared study of the interaction between germ-cell specific sulfogalactosylglycerolipid and dimyristoylglycerophosphocholine

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摘要：

In this study, we investigated structural and dynamic changes of sulfogalactosylglycerolipid (SGG) and dimyristoylglycerophosphocholine (DMPC) in a liposomal system (SGG+DMPC, molar ratio 2:3) by Fourier-transform infrared (FTIR) spectroscopy. Cooling of the preheated SGG liposomes (5–65°C) revealed that the liquid crystalline-to-gel phase transition was centered at 45°C. SGG+DMPC liposomes showed a single phase transition at 28°C. Spectral changes of the ester CO groups of SGG and DMPC in the mixed liposomes indicated a decrease in their interfacial hydrogen bonding intermolecularly and with water. Analysis of SGG's symmetric and antisymmetric CH 2 stretching bands revealed that the insertion of DMPC into SGG bilayers increased the number of gauche conformers in SGG's hydrocarbon chains. Overall, the SGG+DMPC liposomes were homogeneous, with reduced interfacial hydrogen bonding and increased orientational and conformational disorder of SGG's hydrocarbon chains.

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关键词：

Fourier-transform infrared spectroscopy Hydrogen bonding Lipid–lipid interaction Liposomes Phosphatidylcholine Sulfogalactosylglycerolipid

DOI：

10.1016/S0009-3084(00)00147-X

被引量：

127

年份：

2000