Long-term proliferation of mouse primordial germ cells in culture

来自 Nature

喜欢 0

阅读量：

124

作者：

JL Resnick，LS Bixler，L Cheng，PJ Donovan

展开

摘要：

PRIMORDIAL germ cells (PGCs) are first identifiable as a population of about eight alkaline phosphatase-positive cells in the 7.0 days postcoitum mouse embryo 1 . During the next 6 days of development they proliferate to give rise to the 25,000 cells that will establish the meiotic population 2 . Steel factor is required for PGC survival both in vivo 3 and in vitro 4,5 and together with leukaemia inhibitory factor stimulates PGC proliferation in vitro 6 . In feeder-dependent culture, PGCs will proliferate for up to 7 days, but their numbers eventually decline and their proliferative capacity is only a fraction of that seen in vivo 6,7 . Here we report a further factor that stimulates PGC proliferation in vitro , basic fibroblast growth factor (bFGF). Furthermore, bFGF, in the presence of steel factor and leukaemia inhibitory factor, stimulates long-term proliferation of PGCs, leading to the derivation of large colonies of cells. These embryonic germ cells resemble embryonic stem cells, pluripotent cells derived from preimplantation embryos, or feeder-dependent embryonal carcinoma cells, pluripotent stem cells of PGC-derived tumours (teratomas and teratocarcinomas) 8 . To our knowledge, these results provide the first system for long-term culture of PGCs.

展开

关键词：

Germ Cells Cells, Cultured Animals Mice Growth Inhibitors Fibroblast Growth Factor 2 Hematopoietic Cell Growth Factors Stem Cell Factor Interleukin-6 Lymphokines