Rab GTPases: specifying and deciphering organelle identity and function.

Ten years ago, 20 Rab proteins had been identified as organelle-specific GTPases, and two were known to be essential for vesicle targeting in yeast. Today,...

SR Pfeffer - 《Trends in Cell Biology》

Sec6/8 Complex Is Recruited to Cell–Cell Contacts and Specifies Transport Vesicle Delivery to the Basal-Lateral Membrane in Epithelial Cells

In budding yeast, the Sec6/8p complex is essential for generating cell polarity by specifying vesicle delivery to the bud tip. We show that Sec6/8 homologs...

Kent，K，Grindstaff，... - 《Cell》

Ypt and Rab GTPases: insight into functions through novel interactions.

Ypt/Rab GTPases are key regulators of vesicular transport in eukaryotic cells. During the past two years, a number of new Ypt/Rab-interacting proteins have...

N Segev - 《Current Opinion in Cell Biology》

The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5.

How epidermal growth factor receptor (EGFR) signalling is linked to EGFR trafficking is largely unknown. Signalling and trafficking involve small GTPases o...

L Lanzetti，V Rybin，MG Malabarba，... - 《Nature》

Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages

In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dens...

V Claus，A Jahraus，T Tjelle，... - 《Journal of Biological Chemistry》

Specific Contributions of the Small GTPases Rho, Rac, and Cdc42 to Dbl Transformation

Dbl is a representative prototype of a growing family of oncogene products that contain the Dbl homology/pleckstrin homology elements in their primary stru...

R Lin，RA Cerione，D Manor - 《Journal of Biological Chemistry》

Evidence for a Symmetrical Requirement for Rab5-GTP in in Vitro Endosome-Endosome Fusion

Early endosome fusion, which has been extensively characterized using an in vitro reconstitution assay, is Rab5-dependent. To examine the requirement for R...

MA Barbieri，S Hoffenberg，R Roberts，... - 《Journal of Biological Chemistry》

Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast

The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions of cell growth. Tropomyosin localize...

DW Pruyne，Daniel H. Schott and Anthony Bretscher - 《Journal of Cell Biology》

Dynactin-dependent, dynein-driven vesicle transport in the absence of membrane proteins: a role for spectrin and acidic phospholipids.

We reconstituted dynein-driven, dynactin-dependent vesicle transport using protein-free liposomes and soluble components from squid axoplasm. Dynein and dy...

V Muresan，MC Stankewich，W Steffen，... - 《Molecular Cell》

What drives membrane fusion in eukaryotes?

Intracellular membrane fusion is currently explained by two main classes of models, which are evaluated in the light of recent experimental evidence.

A Mayer - 《Trends in Biochemical Sciences》