Counting Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during Viral Infection

来自 Semantic Scholar

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作者：

K Murali-Krishna，JD Altman，M Suresh，DJD Sourdive，AJ Zajac，JD Miller，J Slansky，Rafi Ahmed §

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摘要：

Viral infections induce extensive T cell proliferation in vivo, but the specificity of the majority of the responding T cells has not been defined. To address this issue we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells during acute LCMV infection of mice. Based on tetramer binding and two sensitive assays measuring interferon-γ production at the single-cell level, we found that 50%–70% of the activated CD8 T cells were LCMV specific (2 × 10virus-specific cells/spleen). Following viral clearance, antigen-specific CD8 T cell numbers dropped to 10per spleen and were maintained at this level for the life of the mouse. Upon rechallenge with LCMV, there was rapid expansion of memory T cells, but after infection with the heterologous vaccinia virus there was no detectable change in the numbers of LCMV-specific memory CTL. Therefore, much of the CD8 T cell expansion seen during viral infection represents antigen-specific cells and warrants a revision of our current thinking on the size of the antiviral response.

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关键词：

CD8-Positive T-Lymphocytes T-Lymphocytes, Cytotoxic Animals Mice, Inbred BALB C Mice, Inbred C57BL Mice Lymphocytic Choriomeningitis Peptide Fragments Histocompatibility Antigens Class I Antigens, Viral