The VIP2 receptor: molecular characterisation of a cDNA encoding a novel receptor for vasoactive intestinal peptide.

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作者：

EM Lutz，WJ Sheward，KM West，JA Morrow，G Fink，AJ Harmar

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摘要：

We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel VIP receptor which we have designated the VIP 2 receptor. In situ hybridisation showed that mRNA for this receptor was present mainly in the thalamus, hippocampus and in the suprachiasmatic nucleus.

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关键词：

Vasoactive intestinal peptide (VIP) G protein-linked receptor Rat pituitary Rat olfactory bulb cDNA cloning cAMP, adenosine 3′:5′-cyclic monophosphate cDNA, complementary DNA CGRP, calcitonin gene related peptide CRF, corticotrophin releasing factor dNTP, deoxynucleotide triphosphate DMEM, Dulbecco's modified Eagle's medium rGHRH, rat growth hormone releasing hormone GLP, glucagon-like peptide I mRNA, messenger RNA PACAP, pituitary adenylate cyclase activating polypeptide PCR, polymerase chain reaction PHI, peptide histidine isoleucine PTH, parathyroid hormone RNase, ribonuclease VIP, vasoactive intestinal peptide