Two Epstein-Barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding

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作者：

EA Grogan，WP Summers，S Dowling，D Shedd，L Gradoville，G Miller

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摘要：

We recently identified, by means of cotransformation of LTK-cells, a region of the Epstein--Barr virus (EBV) genome (the BamHI K fragment) that encodes or induces an EBV nuclear neoantigen (EBNA) serologically related to the EBNA found in lymphoid cells carrying the entire EBV genome. We now find that a second EBV DNA fragment, BamHI M, is also able to give rise to cotransformed LTK-cells with stable expression of a nuclear antigen. The BamHI K and M fragments have no apparent DNA homology. Many human sera that are reactive to EBNA in Raji cells detect both antigens; however, certain anti-EBNA-positive human sera are discordant and react only with the BamHI M or only with the BamHI K nuclear antigen. Every Raji cell appears to express both ``M'' and ``K'' antigens; D98 Raji cells, a somatic cell hybrid, express only ``K'' antigen. The K antigen is found on metaphase chromosomes of LTK cells and Raji cells. The M-induced antigen is not located on chromosomes when the cells are in metaphase but is present as granules within the nucleus.

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关键词：

Medical Sciences Chromosomes Nuclear Antigen

DOI：

10.1073/pnas.80.24.7650

被引量：

194

年份：

1983