Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes
摘要：
With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.
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关键词：
Biochemistry Synthetic DNA Globin mRNA RNA-dependent DNA polymerase Oligo(dT)-cellulose Chromatography
DOI：
10.1073/pnas.71.10.3892
被引量：
年份：
1974
掌桥科研
万方医学
Semantic Scholar
dx.doi.org
JSTOR
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