A simplified micro double diffusion agar precipitin test

来自 NCBI

阅读量：

作者：

展开

摘要：

Wadsworth's micro double-diffusion agar precipitin technique is presented as a modified, complete procedure suitable for frequent use in unspecialized clinical and bacteriologic laboratories. This procedure has proved more convenient, and far more sensitive and economical in every sense than conventional Ouchterlony methods. Details are given for setting up the test and for washing, staining, and preserving the precipitin reactions.

展开

关键词：

When tomatoes were planted in steamed soil inoculated with spores of Didymella lycopersici Kleb., a large proportion became infected but very few became infected in unsteamed soil similarly inoculated. In soil treated with formalin and subsequently inoculated the number of diseased plants was about half that in steamed soil. Reinfecting steamed soil with soil micro-organisms before inoculation with D. lycopersici reduced the amount of disease. Covering steamed soil with a layer of maiden loam filtered out a large proportion of D. lycopersici spores applied in water suspension and reduced the amount of disease. Addition of stable manure either before or after steaming did not affect the number of diseased plants Steamed soil remained susceptible to infection by D. lycopersici for 4 months