Relationship of polypeptide products of the transforming gene of Rous sarcoma virus and the homologous gene of vertebrates

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摘要：

All vertebrate cells have been shown to contain a gene, sarc, that has some homology with the transforming gene of Rous sarcoma virus, src. We have compared the polypeptide products of the sarc gene, p60sarc, of human, mouse, and chicken cells with the polymorphic polypeptide product of the src gene, p60src, of several strains of Rous sarcoma virus by two-dimensional peptide mapping. p60sarcfrom chicken cells was clearly related to every viral p60src. Eleven of its 13 methionine-containing tryptic peptides were present in some viral p60src. Conversely, the other two peptides were not present in any p60srcwe have examined so far. The 11 peptides from p60sarcof chickens that were shared with viral p60src, however, were not all present in any single viral p60src. These 11 peptides most closely resemble those in the p60srcs of B77 virus and the Prague strain of Rous sarcoma virus. These data are consistent with the hypothesis that cellular sarc is the progenitor of viral src. The p60sarcs of human, mouse, and chicken cells were so similar in tryptic peptide composition that they were more closely related to each other than were some viral p60srcs. The two mammalian p60sarcs differed from avian p60sarcmost notably in that they lacked a peptide that chicken p60sarcshares with all the viral p60srcs. The similarity of these maps suggests that the sequence of the p60sarcpolypeptide has diverged very little during evolution. This may imply that p60sarcis an essential cellular component.

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关键词：

Cell Biology Src Gene Product Sarc Gene Product Peptide Maps

DOI：

10.1073/pnas.77.4.2059

被引量：

2090

年份：

1980