Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells.

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作者：

C Friesen，I Herr，PH Krammer，Debatin, Klaus-Michael

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摘要：

Cytotoxic drugs used in chemotherapy of and cause apoptosis in target . In lymphoid the CD95 (-1/)/() system is a key regulator of apoptosis. Here we describe that doxorbicin induces apoptosis via the CD95/system in T-lines. -induced apoptosis was completely blocked by inhibition of and . Also, doxorbicin strongly stimulates messenger RNA expression in vitro at concentrations relevant for therapy in vivo. CEM and jurkat resistant to CD95-mediated apoptosis were also resistant to doxorbicin-induced apoptosis . Furthermore, doxorbicin-induced apoptosis was inhibited by blocking F(ab')2 anti--1 (anti-CD95) fragments. Expression of mRNA and in vitro was also stimulated by other cytotoxic drugs such as . The finding that apoptosis caused by anticancer drugs may be mediated via the CD95 system provides a new molecular insight into resistance and sensitivity toward chemotherapy in .

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关键词：

aging nuclear DNA oxidative damage free radical 8-hydroxydeoxyguanosine

DOI：

10.1038/nm0596-574

被引量：

2245

年份：

1996